Volume 29, Issue 1 , May and June 2022, , Pages 101-114
Abstract
Background: Increasing antibiotic resistance of bacteria, especially Escherichia coli, is currently a global health problem. The aim of this study was to investigate the drug resistance pattern of E coli strains isolated from broiler poultry of Ardabil city to common twenty antibiotics in the Iran.Materials ...
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Background: Increasing antibiotic resistance of bacteria, especially Escherichia coli, is currently a global health problem. The aim of this study was to investigate the drug resistance pattern of E coli strains isolated from broiler poultry of Ardabil city to common twenty antibiotics in the Iran.Materials and Methods: Five carcasses were randomly selected from 24 broiler flocks with clinical signs of colibacillosis and sampled from liver and heart using sterile swabs. After culture and isolation, colonies were identified by biochemical and serological methods. Antimicrobial susceptibility testing was performed by disk diffusion method based on CLSI guidelines with antibiotics from various groups.Results: From 120 samples examined, E. coli was isolated in 111 cases (92.5%). The highest antibiotic resistance was in tetrayclin (100%), chlortetracycline (100%), nalidixic acid (100%), oxytetracycline (97.30%), flomequine (94.60%), tylosin (86.47%), difloxacin (83.78%), doxycycline(82.89%), neomycine (81.08%), and sulphamethoxazole+ terimethoprime (74.77%), respectively. The most sensitivity was seen in antibiotics ceftifour (100%), cefixime (89.19%), gentamicin (80.19%) and lincospectin (72.07%), respectively. Multiple antibiotic resistance was in all isolates.Conclusions: The results of this study show the highest rate of drug resistance was observed to common antibiotics in the Iranian poultry industry. This can be due to excessive use of antibiotics in poultry farms. These findings are important for public health and health of human societies.Key words: Escherichia coli, multiple antibiotic resistance, antimicrobial susceptibility testing